The protests disrupted movement of both public and private vehicles, with long queues forming as travellers waited for the situation to stabilise.
Witnesses said protesters gathered in large numbers early in the morning, blocking sections of the road as they expressed their anger over the attack.
Many carried placards and waved twigs while chanting slogans condemning the incident and calling for those responsible to be brought to justice.
The demonstrations intensified as more residents joined, bringing business in the area to a temporary halt.
Traders were forced to close their shops, while commuters were left stranded as public transport operators suspended services due to safety concerns.
Local leaders and community members voiced frustration over the incident, describing it as unacceptable and demanding swift action from authorities.
They urged security agencies to identify and arrest the perpetrators, warning that failure to do so could lead to further unrest.
The attack on Osotsi has sparked widespread concern across the region, with residents expressing fears over rising insecurity.
Many called for increased police presence and more decisive measures to prevent similar incidents in the future.
Efforts by authorities to restore calm were ongoing, with security personnel deployed to monitor the situation and reopen the affected sections of the highway.
By midday, some parts of the road had been cleared, allowing limited traffic to resume under close supervision.
Despite the gradual return to normalcy, tension remained high, with residents insisting that their grievances must be addressed.
The protests highlighted the depth of public anger and the urgency of resolving the matter.
Observers say such demonstrations reflect broader frustrations among communities when incidents of violence involving public figures occur.
The disruption along the Kisumu-Busia highway underscores the economic and social impact of protests, particularly in busy transport corridors.