As a result, traffic built up quickly, especially in the CBD, where movement almost came to a standstill.
Workers, students, and traders were forced to walk long distances or wait for hours to find alternative means of transport.
According to Karakacha, the decision to suspend the strike was made after consultations with stakeholders and appeals from the public.
He said matatu operators understand the suffering caused to ordinary Kenyans and do not wish to punish commuters while discussions are ongoing.
“We have decided to call off the strike until Monday to allow room for dialogue,” he said.
The strike was called to protest what operators described as harsh regulations, high operational costs, and constant harassment on the roads.
Matatu owners and drivers have complained about increased levies, penalties, and enforcement measures that they say are making it difficult to stay in business.
Rising fuel prices and maintenance costs have also added pressure to the sector.
During the strike, police moved in to clear blocked roads in the CBD, towing matatus that had been parked in unauthorized areas.
While authorities said the action was necessary to restore order, it further highlighted the tension between matatu operators and enforcement agencies.
Some operators accused the police of using excessive force instead of seeking dialogue.
Karakacha noted that the suspension of the strike does not mean the issues have been resolved.
He warned that if talks with the government and relevant agencies fail, matatu operators may resume industrial action next week.
He urged authorities to take their concerns seriously and engage them in meaningful discussions.
Commuters welcomed the decision to suspend the strike, saying the situation in the city had become unbearable.
Many described the day as stressful and exhausting, with some arriving at work late or failing to reach their destinations altogether.
Business owners in the CBD also reported losses due to reduced customer traffic.